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          Reagent Center

          Fast Lysis Method

          • BioFast Soil Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC21S1

            Components

            50Tests

            SP Buffer

            45ml

            Lysis S Buffer

            5ml

            DA Buffer

            12.5ml

            Binding Buffer

            35ml

            Wash Buffer

            30ml(add 45ml absolute ethanol before use)

            Elution Buffer

            15ml

            Grind Tube

            50tubes

            Spin Column

            50tubes

            Handbook

            1copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

                At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.

                Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.

            Product Features:
            Storage

            All reagents, when stored properly, are stable for 18 months.

            Apparatus and Materials to Be Supplied by the User

            *  Sterile 2.0ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g              * Absolute ethanol

            Important notes

            • 1. Please add 45ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
            • 2. Lysis S Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target: 2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis

            0.8~1% Agarose gel

            Real-time Taqman PCR detect


            Manual download
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          万搏电竞max-万博电竞max-万博电竞max下载

          
          
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