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          Reagent Center

          Extraction Method

          • BIOZOL Total RNA Extraction Reagent MORE
            Product Details:

             

            Cat#

             

            BSC51S1

             

            BSC51M1

            BIOZOL-total RNA Extraction reagent

            30 ml

            100 ml

            Handbook

            1copy

            1copy

            General information

                BIOZOL Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue or from bacterium. The operation of the kit is very simple and easy. First, add the sample into BIOZOL for complete lyses. Second, add chloroform into the solution and then centrifuge the sample tube. Then the homogenate will separate into form three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.

                Since the operation of BIOZOL is very easy, it can realize the extraction for several samples simultaneously. And total RNA isolated by BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.

            RNA Extraction Procedure

             


            Product Features:

            Storage and Transportation

            ◆   Recommended to store at 2-8℃ and the stability is one year. Recommended to avoid light source so as to achieve optimized effect. Stored at room temperature for a while will not influence the effect.

            ◆   It can be shipped at room temperature which does not affect its stability.

            Important Notes

                RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.

            ◆    Always wear gloves and respirator in order to prevent RNA degradation.

            ◆    Use sterile, disposable plastic ware and automatic pipettes.

            ◆    All plastic wares and tips should be RNase-free as recommended done as below: After washed, metal ware items can be baked at 150°C for 6-8 hours, while  tips or glass and plastic items can be soaked for 2 hours at 37 °C or whole night (12hrs) at room temp in 0.1%(v/v) diethylpyrocarbonate (DEPC) solution, and autoclaved for 20mins in 151bf/in2 (1.034x105 Pa) autoclave and then baked at 60°C.

            ◆    Build a separate RNA laboratory with the only instruments if possible.

            Experimental data:

             

            Total RNA pictures above are strictly operated according to Biozol extraction protocol.


            Manual download
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          万搏电竞max-万博电竞max-万博电竞max下载

          
          
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