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          Reagent Center

          Fast Lysis Method

          • BioFast BIOZOL Total RNA Extraction Reagent MORE
            Product Details:

            Kit Components

            Cat#

            BSC59M1

            Components

            100Tests

             BIOZOL Total RNA Extraction Reagent

            100 ml

             Handbook

             1 copy

             RNA Grind tube

             100 tubes

            Introduction

                BioFast Biozol Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. The operation of the kit is very simple and easy. First, add the sample to the grind tube with Biozol for complete lysis. Second, add chloroform to the solution and then centrifuge the grind tube. The homogenate will be separated to three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.

                Since the operation of BioFast Biozol is very easy, it can realize the extraction  for several samples simultaneously. And total RNA isolated by BioFast BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.

            Product Features:
            Storage and transportation
            •  1. Recommended to store at 2-8 ℃ and the quality guarantee period is up to one year. Recommended to avoid light source so as to achieve optimized effect. Stored at room temperature for a while will not influence the performance of the reagent.
            • 2. The kit can be transported at room temperature.
            Important Notes

                RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.

             

            • ◆Always wear gloves and respirator in order to prevent RNA degradation.
            • ◆Use sterile, disposable plastic ware and automatic pipettes.
            • ◆All plastic wares and tips should be RNase-free as recommended done as below: After washed, metal ware items can be baked at 150°C for 6-8 hours, while tips or glass and plastic items can be soaked for 2 hours at 37 °C or whole night (12hrs) at room temperature in 0.1%(v/v) diethylpyrocarbonate (DEPC) solution, and autoclaved for 20mins in 151bf/in2 (1.034x105 Pa) autoclave and then baked at 60°C.
            • ◆Build a separate RNA laboratory with the only instruments if possible.

             

            Experimental data:
            • Absorbance analysis of yield and purity

            lPrepare RNA, dilute by 25mM Tris-HCl(pH7.5), RNase-free water or TE buffer in a right factor;

            lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer;

            lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

            Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)

            • The conversion factor for RNA is 0.040μg/μl per OD260 unit
            • Notice: the range of Spec reading A2600.1 260<1.0

            lCalculate the purity of RNA as follows: Ratio=(Spec.readingA260)/( Spec.readingA280)

            • Ration of 1.8~2.1 is considered ideal purity.

            (The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.)

            Pictures of Experiment

            Picture 1:Total RNA extracted from animal tissues

            Picture 2 Total RNA extracted from rice leaves

                                

            Manual download
          • BioFast Simply P Total RNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC60S1

            Components

            50Tests

             RNA Grind tube

            50 tubes

            Solution R2

            30ml

            Wash Buffer

            18ml (add 54ml ethanol before use)

             Elution Buffer

            10ml

             Spin columns

            50

            Handbook

             1copy

            Introduction

                Biofast-simplyP is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. Add sample to the grind tube with Solution R2 for homogenization and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.

                The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-through put. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+  selection、in  vitro  translation、RNase  protect  assay,  RT-PCR/Real  time  RT-PCR analysis、construction cDNA library etc.

            Product Features:

            Storage and transportation

            •  1. The kit has demonstrated stability of 24 months when stored at room temperature.
            •  2. The kit can be transported at room temperature.

            Apparatus and materials to be prepared by the user

            * Sterile 1.5ml microcentrifuge tubes             * 10µl/200µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm           * Absolute ethanol        *  Vortex mixer

            Important note

            Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

            Experimental data:

            Analysis RNA

            • Absorbance analysis of yield and purity

            lPrepared RNA will be dilute by 25mM Tris-HCl (pH7.5), RNase-free water or TE buffer in a right factor;

            lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl(pH7.5), RNase-free water or TE buffer;

            lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

            Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)

            1. The conversion factor for RNA is 0.040μg/μl per OD260 unit

            2. Notice: the range of Spec reading A260: 0.1≤OD260≤1.0

            Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/( Spec.readingA280)

            3. Ration of 1.8~2.0 are considered ideal purity.

            The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.

            Picture 1:Animal tissues                                     

                       

            Picture 2:Rice leaf

                     


            Manual download
          • BioFast Soil Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC21S1

            Components

            50Tests

            SP Buffer

            45ml

            Lysis S Buffer

            5ml

            DA Buffer

            12.5ml

            Binding Buffer

            35ml

            Wash Buffer

            30ml(add 45ml absolute ethanol before use)

            Elution Buffer

            15ml

            Grind Tube

            50tubes

            Spin Column

            50tubes

            Handbook

            1copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

                At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.

                Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.

            Product Features:
            Storage

            All reagents, when stored properly, are stable for 18 months.

            Apparatus and Materials to Be Supplied by the User

            *  Sterile 2.0ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g              * Absolute ethanol

            Important notes

            • 1. Please add 45ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
            • 2. Lysis S Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target: 2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis

            0.8~1% Agarose gel

            Real-time Taqman PCR detect


            Manual download
          • BioFastSpin Plant Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC18S1

            Components

            50Tests

            LP Buffer

            45 ml

            DA Buffer

            10 ml

            P Binding Buffer

            16ml

            G Binding Buffer

            25ml

            Wash Buffer

            25ml

            Elution Buffer

            10ml

            Spin Column

            50

            Grind Tube

            50

            Handbook

            1 copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from plant tissues, Designed for use with the lysis instrument from BIOER,plants are easily lysed within 40 s.The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this Kit.

                The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

            Product Features:
            Storage
            • 1. The kit should be stored at 15-25℃.
            • 2.  All reagents, when stored properly, are stable for 18 months.
            Apparatus and Materials to Be Supplied by the User

            *  Sterile 1.5ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g              * Absolute ethanol            * Vortex mixer

            Important notes
            • 1. Please add 37.5ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
            • 2. Please add 32ml absolute ethanol to P Binding Buffer and mix thoroughly before the first use.
            • 3. LP Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Get some DNA,diluted in a advisable factor with Elution Buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target:   2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis

            0.8~1% Agarose gel

            Example 1:Rice leaves

            Example 2:Rice leaves


            Manual download
          • BioFastSpin Tissue Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC20S1

            Components

            50Tests

            FL Buffer

            30ml

            Binding Buffer

            35ml

            PW Buffer

            10.5ml

            Wash Buffer

            25.2ml

            Elution Buffer

            10ml

            PK Solution

            0.5ml

            WS buffer

            5ml

            Grind Tube

            50 tubes

            Spin Column

            50 tubes

            Handbook

            1 copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than1.5 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 30 μg genomic DNA can be acquired from up to 50 mg tissue by using this kit.

                The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

            Product Features:
            Storage
            • 1. The Protease K is to be stored at 2-8℃, others at 15-25℃.
            • 2. All reagents, when stored properly, are stable for 18 months.

            Apparatus and Materials to Be Supplied by the User

            *  Sterile 2.0ml microcentrifuge tubes                    * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g                 * Absolute ethanol

            Important notes

            • 1. Please add 15.75ml absolute ethanol to PW Buffer and mix thoroughly before the first use.
            • 2. Please add 37.8ml absolute ethanol to wash Binding Buffer and mix thoroughly before the first use.
            • 3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37℃ until the precipitate has fully dissolved.
            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target:    2.1≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis

            0.8~1% Agarose gel

            Example 1:Mouse heart and mouse muscle

            B: Bioer Kit

            O: Other company Kit

            Example 2:Mouse liver


             

            Manual download
          Privacy clause

          Respect the user's privacy is a basic policy of Hangzhou Bioer user service. Hangzhou Boier will not disclosure user information to third parties or disclose their registration and preservation of non disclosure in Hangzhou Bioer platform, except in the following situations: (1)User's improper behavior caused leakage of privacy information ; (2)Caused by network, hacker attack, computer virus, government regulation, the reasons of the leakage, lost, stolen or tampered with other relevant legal requirements; (3)Law-enforcement agency asked Hangzhou Bioer to provide privacy information of the user; (4)Emergency situation when public life and property security is under threate.

          Exemption clause

          Hangzhou Bioer technology does not guarantee the accuracy, integrity and reliability of any content on this website and any content that may use these results. Hangzhou Bioer technology and related people can not guarantee that you can enter, browse and use this website at any time; Hangzhou Bioer technology on this website and its contents can not use and do not take any responsibility. The site links to other websites that Hangzhou itself is not recognized or undertake other Bioer technology content or the use of responsibility. Hangzhou Bioer technology and related people to enter, browse and use of the site, from the website to download any content or by any link to this site to other sites caused by viruses or other destructive programs on your computer system and any other software and hardware, IT system or property damage or loss does not bear any responsibility. Hangzhou Bioer technology and the relevant people do not bear any responsibility caused by the third party using illegal means to obtain the password, data and content into the site of any direct and indirect damage or loss . The final interpretation belong to Hangzhou Bioer Technology Co. Ltd.

          Hangzhou Bioer reserves the right to correct, modify and update this statement at any time.

          Copyright

          This website from Hangzhou Bioer Technology Co. Ltd. (hereinafter referred to as "Hangzhou Bioer") the creation of any content of this website (including but not limited to text, data, graphics, images, voice or video) are copyright or related rights belong to Hangzhou Bioer. Hangzhou Bioer or related rights without prior written permission, you shall not in any way without permission to copy, reconstruction, spread, publishing, reprint, repost or display the contents of this website. Any unauthorized use of this site may violate the "Copyright Law of People's Republic of China " and other relevant laws and regulations and international conventions, Hangzhou Bioer fully pursue appropriate legal responsibility right.

          Contact Us

          Address: 1192 Bin An Rd, Hi-tech (Binjiang)District,Hangzhou,310053,P. R. China

          Tel:0571-87774567

          万搏电竞max-万博电竞max-万博电竞max下载

          
          
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