"

万搏电竞max-万博电竞max-万博电竞max下载β拥有全球最顶尖的原生APP,每天为您提供千场精彩体育赛事,万搏电竞max-万博电竞max-万博电竞max下载β更有真人、彩票、电子老虎机、真人电子竞技游戏等多种娱乐方式选择,万搏电竞max-万博电竞max-万博电竞max下载β让您尽享娱乐、赛事投注等,且无后顾之忧!


<address id="lxprp"></address>

<address id="lxprp"></address>

<form id="lxprp"></form>

        <address id="lxprp"><nobr id="lxprp"><meter id="lxprp"></meter></nobr></address>

        <em id="lxprp"><form id="lxprp"></form></em>
          <noframes id="lxprp"><listing id="lxprp"></listing>

          <address id="lxprp"><listing id="lxprp"><menuitem id="lxprp"></menuitem></listing></address>

          "

          Reagent Center

          Column Method

          • Simply P Virus RNA Extraction Kit MORE
            Product Details:

            Main constituents

             

            Cat#

            BSC56S1

            BSC56M1

            BSC56L1

             

            Ingredients

            Kit Content

            50T

            100T

            200T

            Lysis Buffer

            25 ml

             50 ml

            100 ml

            Salt and Tris-HCl Buffer

            Wash Buffer I

            18ml

            36ml

            72ml

            High-salt solution

            Wash Buffer II

            12ml

            24ml

            48ml

            Low-salt solution

            RElution Buffer

            10ml

             20ml

            40ml

            RNase-free H2O

            Spin Columns

            50

             100

            200

            Plastic parts and nucleic acid adsorption film

            Handbook

            1

             1

            1

             

            PSBuy BSC56S add 12ml Absolute ethanol to 18ml Wash Buffer I before use. add 48ml Absolute ethanol to 12ml Wash buffer II before use.

            Buy BSC56M1 add 24ml Absolute ethanol to 36ml Wash Buffer I before use. add 96ml Absolute ethanol to 24ml Wash buffer II before use.

            Buy BSC56L1 add 48ml Absolute ethanol to 72ml Wash Buffer I before use. add 192ml Absolute ethanol to 48ml Wash buffer II before use.

            Product Features:

            Reagent prepared by the user

            Absolute ethanol (AR).

             Storage and transportation

            1)  The kit can be transported at room temperature.

            2)  The kit has demonstrated stability of 12 months when stored at room temperature.

            Notice

            1)  Lysis Buffer may be precipitated at low temperature, please heated at 56 ℃ for a few minutes to restore the clarification.

            2)  Wash Buffer I and Wash Buffer II add the absolute ethanol as the volume marked on bottle label and mix well.

            Experimental data:

            Purification coronavirus RNA from cat ascites by this kit, real-time PCR result.

            Manual download
          • Simply P Total RNA Extraction kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC52S1

            BSC52M1

            Components

            50Tests

            100Tests

            Solution R1

            10ml

            20ml

            Solution R2

            30ml

            60ml

            Wash Buffer

            25ml

            (add 75ml ethanol before use)

            25ml×2

            (add 75 ml ethanol before use)

            Elution Buffer

            10ml

            20ml

            Spin columns

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit is a ready-to-use reagent for the isolation of total RNA from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. Add Solution R2 to the processed sample and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+ selection、in vitro translation、RNase protect assay, RT-PCR/Real time RT-PCR analysis ,construction cDNA library etc.

            Product Features:

            Storage and transportation

            •  The kit has demonstrated stability of 24 months when stored at room temperature.
            •  The kit can be transported at room temperature.

            Technical Information

            Method

            Time

            Max volume

            Yield

            Spin column

            7~15min

            750µl

            ≥90%

            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm                          * Absolute ethanol

            *  Vortex mixer

            Important note

            Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

            Experimental data:

            Analysis RNA

            Absorbance analysis of yield and purity

            lPrepare RNA, dilute by 25mM Tris-HCl(pH7.5), RNase-free water or TE buffer in a right factor;

            lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer;

            lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

            Final concentration = (Spec reading A260) × (Dilution factor)  × (Conversion factor A260)

            ð The conversion factor for RNA is 0.040μg/μl per OD260 unit

            ð Notice: the range of Spec reading A2600.1≤OD260≤1.0

            Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/ (Spec.readingA280)

            ð Ration of 1.8~2.0 are considered ideal purity.

            The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.

            Picture 1:Animal

            Manual download
          • Biospin Whole Blood Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC06S1

            BSC06M1

            Component

            Amount

            Amount

            PK solution

            0.5ml

            1.0 ml

            LysisB Buffer

            10.0ml

            20.0 ml

            WB1 Buffer

            11.825ml

            23.65ml

            Wash Buffer

            11.55ml×2

            23.1ml×2

            Elution Buffer

            10ml

            20ml

            Spin column

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit provide a very simple, fast and effective technique for the isolation of pure high–molecular-weight genomic DNA in whole blood which was treated with anticoagulants such as citrate, heparin,  EDTA. The kit is also suitable for the extraction of genome DNA from leukocytes. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely  avoided.In  general,  2~6μg  genomic  DNA  can  be  acquired  from 200μl blood using the kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage

            • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            • All components, when stored properly, can keep stable for 18 months.

            Apparatus and Materials should Be Supplied by the User

            *   1.5ml sterile Micro-centrifuge tubes        * 10µl/100µl/1000µl tips

            *   Micro-centrifuge capable of 14,000×g    * Absolute ethanol (>99%)

            *   Vortex mixer

            Experimental data:

            Analysis DNA

            •  Absorbance analysis

            Take some DNA,dilute it into an appropriate concentration with elution buffer.

            Measure it at   OD260   ,OD280  and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple Target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            •  Agarose Gel Analysis

            0.8~1% Agarose gel

            Example 1:Blood

            Manual download
          • Biospin Yeast Plasmid DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC01S1G

            BSC01M1G

            Components

            50Tests

            100Tests

            RS Buffer

            30.0ml

            60.0ml

            Resuspension Buffer

            12.5ml

            25ml

            Lysis Buffer

            12.5ml

            25ml

            Neutralization Buffer

            17.5ml

            35ml

            PW Buffer

            25ml

            50ml

            Wash Buffer

            13.0ml

            (add 52ml ethanol before use)

            26.0ml

            (add 104ml ethanol before use)

            Snailase

            260mg×2 (2-8℃)

            260mg×4(2-8℃)

            RNase A

            50ul  (2-8℃)

            100ul (2-8℃)

            Elution Buffer

            10.0ml

            20.0ml

            Spin columns

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The Kit provides a fast, simple, and cost-effective yeast plasmid miniprep method for routine  molecular biology laboratory applications. Yeast plasmid DNA can be purified from 3–5ml of overnight cultures of yeast. The DNA isolated by this Kit is ready for downstream applications such as transformation, sequencing, PCR/Real-time PCR and other downstream experiments.

            Product Features:

            Storage and Transportation

            • The kit should be stored at room temperature(15~25℃), but the Snailase and the RNase A should be stored at 2~8℃,the Snailase solution should be stored at -20℃.The kit can be stored for up to 12 months by this method. After addition of RNase A, Resuspension Buffer should be stored 2~8℃.
            • The kit can be transported at room temperature.

            Materials to Be Supplied by the User

            * Sterile 1.5ml micro centrifuge tubes     * Absolute ethanol     *β-mercaptoethanol

            Important notes

            1. Please use 1.3 ml RS Buffer to oscillation dissolve each tube snailase, then hold - 20 ℃.
            2. The β-mercaptoethanol should be added into the RS Buffer (10ul/ml) before use. This mixture is  good at room temperature for 1 week.
            3. The RNase A should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
            4. Add ethanol (as the volume is marked on bottle label) to Wash Buffer then mix well.
            5. If the Lysis Buffer and Neutralization Buffer precipitated, it should be redissolved by warming to 37°C. Please not vortex Lysis Buffer acutely.
            6. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
            7. The Kit can extract high-quality yeast plasmid DNA from 3-5ml yeast culture overnight cultured.
            Experimental data:

            DNA Analysis

            • Absorbance analysis

            Get some plasmid DNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target: 2.0≥OD260-320/ OD280-320≥1.8

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis
              0.8~1% Agarose gel

            Example 1:Plasmid DNA electrophoresis

            DL15, 000                                    DL2, 000

            Example 2:Enzymatic reactions analysis

                 DL2, 000                                                                                                         DL15, 000

            Manual download
          • Biospin Whole Blood Genomic DNA Midi Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC06S1B

            Components

            10Tests

            PK solution

            1.5ml

            Balance Buffer

            10ml

            5×RCL Buffer

            60ml

            NS Buffer

            20ml

            LysisB Buffer

            20ml

            WB1 Buffer

            17.2ml

            (add22.8ml ethanol before use)

            Wash Buffer

            21ml

            (add 49ml ethanol before use)

            Elution Buffer

            20.0ml

            MidiSpin column

            10

            Handbook

            1 copy

            Introduction

                The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood which was treated with anticoagulants such as citrate, heparin, EDTA. Based on the remarkable selectivity on genome DNA of Midispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic   DNA within 2hours. No any expensive equipment are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. In general, 150~250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、 Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage

            • The PK solution and 5×RCL Buffer must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            •  All components, when stored properly, can keep stable for 18months.

            Apparatus and Materials should Be Supplied by the User

            *  50ml sterile Micro-centrifuge tubes                 * 10µl/100µl/1000µl tips

            *  Micro-centrifuge capable of 5,000×g               * Absolute ethanol (>99%)

            *  Vortex mixer                                                 * warm bath

            Before Starting:

            (1)     According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.

            (2)       Add 4 volumes of deionized water to 1 volume of 5×RCL Buffer.

            (3)      Add 1ml Balance Buffer to each MidiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Take some DNA,dilute it into an appropriate concentration with elution buffer. Measure it at OD260,OD280 and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple

            target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            •  Agarose Gel Analysis

            0.8~1% Agarose gel

            Manual download
          • Biospin Whole Blood Genomic DNA Maxi Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC06S1C

            Components

            10Tests

            PK solution

            5ml

            Balance Buffer

            20ml

            LysisB Buffer

            70ml×2

            WB1 Buffer

            25.8ml

            (add 34.2ml ethanol before use)

            Wash Buffer

            64ml

            (add 96ml ethanol before use)

            Elution Buffer

            20.0ml

            MaxiSpin column

            10 tubes

            Handbook

            1 copy

            Introduction

                The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in 10ml whole blood based on the remarkable selectivity on genome DNA of Maxispin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 2hours. No any expensive equipment are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general, 150~250μg genomic DNA can be acquired from 10ml blood using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage

            • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            • All components, when stored properly, can keep stable for 18months.

            Apparatus and Materials should Be Supplied by the User

            *  50ml sterile Micro-centrifuge tubes       * 10µl/100µl/1000µl tips

            *  Micro-centrifuge capable of 5,000×g     * Absolute ethanol (>99%)

             *  Vortex mixer                                      * warm bath

            Before Starting:

            1. According to the label on the bottle, add ethanol to WB1 buffer and Wash buffer.
            2. Add 2 ml Balance Buffer to each MaxiSpin column, centrifuge at 5,000 x g for 3 min. Discard flow-through.

            Note:Each elution typically yields 60%of DNA bound to the column. To obtain DNA at higher concentrations, please suck the liquid collected in the collection tube again to the column. Incubate at room temperature for 2 minutes. Centrifuge at 4,000×g for 3 minutes. The DNA in the collection tube is ready for further analysis. If the isolated DNA sample is not going to be tested on the same day, freeze it at -20℃.

            Experimental data:

            Analysis DNA

            •  Absorbance analysis

            Take some DNA,dilute it into an appropriate concentration with elution buffer. Measure it at OD260,OD280 and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple

            target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            • Agarose Gel Analysis 

            0.8~1% Agarose gel

            Example 12ml whole blood


            Example 25ml Blood clots


            Manual download
          • Biospin Virus RNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC62S1

            BSC62M1

            Components

            50Tests

            100Tests

            RLysis Buffer

            15ml

            30ml

            RD Buffer

            17.5ml

            35ml

            DNase Stop Buffer

            5.0ml

            (add 6.5ml ethanol before use)

            10.0ml

            (add 13ml ethanol before use)

            Wash Buffer

            20ml

            (add 30ml ethanol before use)

            40ml

            (add 60ml ethanol before use)

            RElution Buffer

            10ml

            20ml

            Spin Columns

            50

            100

            Handbook

            1copy

             1copy

            Introduction

                The kit is a ready-to-use reagent for the isolation of Virus RNA from different type’s sample. Add RLysis Buffer to the processed sample and adding alcohol will bind RNA to spin column. Then RNA can be easily isolated through several washing and eluting steps.

                The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation, RNase protect assay, RT-PCR/Real-time RT-PCR analysis, construction cDNA library etc.

            Product Features:

            Storage and transportation

            • The kit has demonstrated stability of 24 months when stored at room temperature.
            • The kit can be transported at room temperature.

            Technical Information

            Sample

            Amount

            Animal tissue

            ≤30mg

            Culture cells

            ≤1×108

            White blood cells

            ≤from 5ml whole blood

            For liquid sample

            ≤100μl

            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml microcentrifuge tubes            * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm       * 70℃ Water bath      * Vortex mixer

            *  Liquid nitrogen (or ice bath)     * Optional: β-Me        * PBS          *Trypsination

            *  Red Blood Cells Lysis Buffer(Cat#BSA06M1)         * Absolute alcohol

            Important note

            • RD Buffer may be precipitated at low temperature, the heated at 37 ℃ for a few minutes, to restore the clarification.
            • DNase Stop Buffer Add the alcohol as the volume marked on bottle label and mix well.
            • Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.
            Experimental data:

            Analysis RNA

            ExampleHCV Real-time RT-PCR  (Sample HCV concentration:copies/ml)

            Manual download
          • Biospin Total RNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC63S1

            BSC63M1

            Components

            50Tests

            100Tests

            RLysis Buffer

            8.75ml

            17.5ml

            RD Buffer

            17.5ml

            35ml

            DNase Buffer

            2ml

            4ml

            MnCl2

            450ul

            900ul

            DNase I

            50ul(stored at -20℃)

            100ul(stored at -20℃)

            DNase Stop Buffer

            5 ml

            (add 6.5 ml ethanol before use)

            10 ml

            (add 13 ml ethanol before use)

            Wash Buffer

            32 ml

            (add 48ml ethanol before use)

            32 ml

            (add 48ml ethanol before use)×2

            RElution Buffer

            10ml

            20ml

            Spin columns

            50

            100

            Handbook

            1 copy

            1 copy

            Introduction

                The kit is a ready-to-use reagent for the isolation of total RNA from animal tissues, cells, bacteria and others (plant tissues are not recommended). Add RLysis Buffer to the processed sample.RD buffer will remove the protein, then adding alcohol will bind RNA to spin column. The DNA will be destroyed by the DNase I reaction. Then RNA can be easily isolated through several washing and eluting steps.

                The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation,RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library etc.

            Product Features:

            Storage and transportation

            • The kit has demonstrated stability of 18 months when the DNase I should be stored at -20 ℃, others at room temperature.
            • The kit can be transported at room temperature.

            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml centrifuge tubes                       * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm           * Absolute alcohol

            *  Vortex mixer                                              * β- mercaptoethanol

             Important note

            • DNase Stop Buffer: Add the alcohol as the volume marked on bottle label and mix well.
            • Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.
            Experimental data:

            Analysis RNA

            • Absorbance analysis

            Get some RNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=40×OD260×dilution fact

            Target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis

            Manual download
          • Biospin SwabGen DNA Extraction Kit MORE
            Product Details:

             Kit Components

             

            Cat#

            BSC28S1

            BSC28M1

            Components

            50Tests

            100Tests

            PK solution

            500ul

            1000ml

            LysisB Buffer

            30ml

            60ml

            WB1 Buffer

            12ml

            (add 17ml ethanol before use)

            24ml

            (add 34ml ethanol before use)

            Wash Buffer

            18ml

            (add 42ml ethanol before use)

            36ml

            (add 84ml ethanol before use)

            Elution Buffer

            10 ml

            20 ml

            Spin column

            50 tubes

            100 tubes

            Handbook

            1copy

            1copy

            Introduction

                The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in Oral swabs. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general,   0.5~3.5μg genomic DNA can be acquired using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.

            Product Features:

            Storage and transportation

            • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            • All components, when stored properly, can keep stable for 18months.
            • The kit can be transported at room temperature.

            Apparatus and materials to be prepared by the user

            *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

            *  Micro-centrifuge capable of 12,000×g                * Absolute ethanol (>99%)

            *  Vortex mixer                                                  * Warm bath or metal bath

            Important note

            Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

            Experimental data:

            Analysis DNA

            •  Absorbance analysis

             Take some DNA,dilute it into an appropriate concentration with elution buffer.

             Measure it at OD260,OD280 and OD320.

            Equation:concentration(μg/ml)=50×OD260×dilution multiple

            target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is reliable.

            • Agarose Gel Analysis 0.8~1% Agarose gel

             SYBR Green Real-time PCR

            Manual download
          • Biospin Polyacrylamide Gel DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC15S1

            BSC15M1

            Components

            50Tests

            100Tests

            PE Buffer

            10 ml

            20 ml

            PEB Buffer

            20 ml

            40 ml

            PWash Buffer

            18.9ml

            37.8ml

            Elution Buffer

            10ml

            20ml

            Spin Column

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit provides a simple, rapid and effective method for purification of DNA fragments from polyacrylamide gel. The simple purification procedure is based on the remarkable selectivity of Biospin membrane. There need few steps to finish extraction without expensive equipment, as completely avoids using toxic and hazardous reagents such as phenol and chloroform. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, PCR, restriction enzyme digestion, and so on.

            Product Features:

            Storage and transportation

            • The kit should be stored dry at room temperature(15~25℃), The kit can be stored for up to 18 months if all components are kept properly.
            • The kit can be transported at room temperature.

            Technical Information

             Method

            Column volume

            DNA size range

            Electrophoresis buffer

            Incubate temperature

            Elution recovery

            Spin column

                 750µl

            100bp~10kb

            TAE/TBE buffer

            65℃

            ≥99%

            Apparatus and Materials to Be Supplied by the User

            * Sterile 1.5 or 2.0 ml microcentrifuge tubes        * 10µl/100µl/1000µl tips

            *  Absolute ethanol    * Microcentrifuge capable of 14,000g * Vortex mixer   * Water bath or cooling & heating block

            Preparation

             

            1. This product cannot be used with PAGE with urea.
            2. Add ethanol as per the volume marked on label of bottle into Wash Buffer and then mix well.
            3. If the gel slice is very big, please increase the dosage of PE Buffer properly but on more than 200μl, thus to guarantee that the gel slice is immerged completely.
            4. The suitable volume of elution buffer for the kit is 50µl. The dosage of elution buffer can be adjusted according to practical experimental situation.
            5. Please wash the fixed gel by water or 10mM Tris-HCl (pH7.0) for 2 or 3 times if the gel is fixed by acidic reagent such as acetic acid.
          • Biospin Plasmid DNA Maxi Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC01S1C

            BSC01M1C

            Components

            10Tests

            25Tests

            Balance Buffer

            20ml

            50ml

            Resuspension Buffer

            100ml

            250ml

            Lysis Buffer

            100ml

            250ml

            Neutralization Buffer

            120ml

            100ml

            Washing Buffer

            50mlх2

            (add 75ml ethanol before use)

            104mlх2

            (add 156ml ethanol before use)

            Elution Buffer

             60ml

            150ml

            RNase A

            1 tube

            1 tube

            Max Spin column

            10 tubes

            25 tubes

            Handbook

            1 copy

            1 copy

            Introduction

                 This kit allows the high efficient binding of DNA to our spin matrix while proteins and  other  impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer. This kit is designed for fast and efficient purification of plasmid DNA from 150 to 200 ml of E. coli culture. The maxi column has a plasmid DNA binding capacity of 1 mg.

                The purified plasmid DNA is ready for downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.

            Product Features:

            Storage and transportation

            •  The kit has demonstrated stability of 18 months when the RNase A should be stored at 2-8℃,others at room temperature.
            • The kit can be transported at room temperature.
            •  Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

            Apparatus and materials to be prepared by the user

             *  50ml centrifuge tubes                            * 10µl/100µl/1000µl tips

             *  Centrifuge capable of 14,000g               * Absolute alcohol

            Important note

            1)Spin down RNase A vial briefly. Add the RNase A solution to Resuspension Buffer and mix well before use. Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.

            2)Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.

            3)Lysis Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

            4)Neutralization Buffer may form precipitates below 10°C, warm up at 37°C to dissolve the precipitates before use.

            5)Carry out all centrifugations at room temperature.

            6) Add 2ml Balance Buffer to each MaxiSpin column, incubate at room temperature for 2 mins,centrifuge at 5,000 x g for 3 min. Discard flow-through. It can improve the yield of DNA significantly.

          • Biospin Plasmid DNA Extraction Kit MORE
            Product Details:

            Kit Components 

            Cat#

             

            BSC01M1

             

            BSC01L1

            Components

            100Tests

            250Tests

            Resuspension Buffer

            25ml

            62.5ml

            Lysis Buffer

            25ml

            62.5ml

            Neutralization Buffer

            35ml

            87.5ml

            Wash Buffer

            30 ml

            (add 45ml absolute ethanol before use) ×2

            75 ml

            (add 112.5ml absolute ethanol before use) ×2

            Elution Buffer

            20ml

            50ml

            RNase solution

            1

            1

            Spin columns

            100

            250

            Handbook

            1copy

            1copy

            Introduction

                The kit provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. Plasmid DNA can be purified from 1–5ml of overnight cultures of E. coli. The DNA isolated by this kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-time PCR and other downstream experiments.

            Product Features:

            Storage

            1.The kit should be stored at room temperature (15~25℃), but the RNase solution should be stored at 2~8℃.The kit can be stored for up to 18 months by this method. After addition of RNase solution, Resuspension Buffer should be stored 2~8℃.

            2.The kit can be transported at room temperature.

            Technical Information

             

            Method

             

            Work time

             

            Column volume

             

            Column yield

             

            Elution recovery

             

            Plasmid DNA length

             

            Culture volume

            Spin column

            25 min for

            24 samples

            750µl

            20µg DNA

            ≥99%

            ≤10kB

            1~5ml high-copy plasmid

            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000 × g            * Absolute ethanol          * Vortex mixer

            Important notes

            1. The RNase solution should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
            2. Add ethanol (as the volume is marked on bottle label) to Wash Buffer and mix well.
            3. If the Lysis Buffer and Neutralization Buffer precipitated, it should be Redissolved by warming to 37°C. Please not vortex Lysis Buffer acutely.
            4. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
            5. The kit can extract high-quality plasmid DNA from 1-5ml E.coli overnight cultured.
            6. The suitable volume is 50ul for Elution Buffer; user can adjust its volume if necessary.
            Experimental data:

            DNA Analysis

            • Absorbance anlysis,

            Get some plasmid DNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target:  2.0≥OD260-320/ OD280-320≥1.8

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis,0.8~1% Agarose gel 

             

            Example 2:Enzymatic reactions analysis

            Manual download
          • Biospin Plasma Circulating DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC30S1

            BSC30M1

            Components

            50Tests

            100Tests

            Solution  Ⅰ

            20ml

            40ml

            Solution  Ⅱ

            10ml

            20ml

            PK solution

            500ul  (Store at 2~8℃)

            1000ul  (Store at 2~8℃)

            LysisB Buffer

            10ml

            20ml

            WB1 Buffer

            12ml

            (add 17ml ethanol before use)

            24ml

            (add 34ml ethanol before use)

            Wash Buffer

            18ml

            (add 42ml ethanol before use)

            36ml

            (add 84ml ethanol before use)

            Elution Buffer

            10 ml

            20 ml

            Spin column

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit provides a very simple, fast and effective technique for the isolation of pure plasma DNA. DNA in the sample is released using PK solution and LysisB Buffer. The released DNA is bound exclusively and specifically to the Biospin membrane in presence of LysisB Buffer and ethanol under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed with  twice washing procedures. The DNA is then eluted from the membrane with the elution Buffer. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. The pure DNA can be applied extensively in PCR/Real-time PCR, and so on.

            Product Features:

            Storage and transportation 

            • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
            •  All components, when stored properly, can keep stable for 18months.
            •  The kit can be transported at room temperature.

            Apparatus and materials to be prepared by the user

            *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

            *  Micro-centrifuge capable of 12,000×g                   * Absolute ethanol (>99%)

            *  Vortex mixer                                                                 * Warm bath or metal bath

            Important note

            Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

          • Biospin PCR Purification Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC03S1

            BSC03M1

            Components

            50Tests

            100Tests

            Binding Buffer

            10ml

            20ml

            Wash Buffer

            15ml

            30ml

            Elution Buffer

            10ml

            20ml

            Spin column

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit provides a simple, rapid and effective method for purification of DNA fragments from PCR or enzymatic reaction. DNA fragments ranging from 60bp to 10kb can be purified. The yield of DNA with size lower than 100bp is 23~95%, while the yield of DNA with size from 0.1kb to 10kb is 90~97%. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion, PCR/real-time PCR and so on.

            Product Features:

            Storage and Transportation

            • The kit should be stored dry at room temperature (15~25℃), The kit can be stored for up to 18 months if all components are kept in the manner above.
            • The kit can be transported at room temperature.

            Apparatus and Materials to Be Supplied by the User

            *  Sterile 1.5 microcentrifuge tubes          * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g     * Vortex mixer     * Absolute ethanol

            Important notes

            1. Add ethanol (as the volume be marked on bottle label) to Wash Buffer and mix well
            2. Close the lid after using the Binding Buffer as soon as possible.
            3. The suitable volume is 50ul for Elution Buffer; user can adjust its volume if necessary.
            Experimental data:

            Analysis DNA

            • Absorbance anlysis

            Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target: 2.0≥OD260-320/ OD280-320≥1.8

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis 0.81Agarose gel

            Example 1:

            According to the absorbance and agarose gel analysis,the impurity had been discarded.

            U: unpurified      P: purified               M: Marker

            Example 2:

            Example3:Elution Volume versus DNA Yield

             

            Manual download
          • Biospin Omni Plant Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC13S1B

            Components

            50Tests

            LP Buffer

            22.5 ml

            LP plus Buffer

            22.5 ml

            DA Buffer

            7.5 ml

            P Binding Buffer

            14ml

            G Binding Buffer

            25ml

            Wash Buffer

            25.2ml

            Elution Buffer

            10ml

            Spin column

            50

            Shredder Spin Column

            50

            Handbook

            1 copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of pure high– molecular-weight genomic DNA from plant tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and  hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this kit.

                The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

             

            Product Features:

            Storage

            • The kit should be stored at 15-25℃.
            •  All reagents, when stored properly, are stable for 18 months.

            Apparatus and Materials to Be Supplied by the User

            *  Sterile 1.5ml microcentrifuge tubes             * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g              * Absolute ethanol            * Vortex mixer

            Important notes

            1. Please add 37.8ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
            2. Please add 28ml absolute ethanol to P Binding Buffer and mix thoroughly before the  first use.
            3. LP Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
            Experimental data:

            Analysis DNA

            Absorbance anlysis

            Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            expressions:concentration(μg/ml)=50×OD260×dilution fact

            target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis 0.81Agarose gel

            Example 1:Rice leaves

            Example2: The extraction of plant tissues rich in polysaccharides and polyphenols

                              

            Manual download
          • Biospin miRNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC64S1

            BSC64M1

            Components

            50Tests

            100Tests

            BIOZOL Reagent

            35ml

            70ml

            Wash Buffer

            12ml

            (add 48ml ethanol before use)

            24ml

            (add 96ml ethanol before use)

            Reslution Buffer

            15ml

            30ml

            Spin columns

            50

            100

            Handbook

            1copy

            1copy

            Introduction

                The kit is a ready-to-use reagent for the isolation of total RNA,including miRNA and other small RNA molecules, from cultured cells and various animal and human tissues. Add BIOZOL Reagent to the processed sample, after addition of chloroform, RNA will be isolated from DNA and Protein, and adding alcohol will bind RNA all RNA molecules from 18 nucleotides (nt) upwards to spin column. Then RNA  can be easily isolated through several washing and eluting steps.

                The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library, microarray analysis etc.

            Product Features:

            Storage and transportation

            • The kit has demonstrated stability of 12 months when the BIOZOL Reagent should be stored at 2-8  ℃,others at room temperature.
            • The kit can be transported at room temperature.
            Apparatus and materials to be prepared by the user

            *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000rpm                  * Absolute alcohol

            *  Vortex mixer                                                      * Chloroform

            Important note

            Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.

            Experimental data:

            Analysis RNA

            • Absorbance analysis

            Get some RNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320.

            Expressions:concentration(μg/ml)=40×OD260×dilution fact

            Target: 2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Real-Time PCR Analysis (Rat rno-miR-21)

            Manual download
          • Biospin Marine Animal Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components

            Cat#

            BSC27S1

            BSC27M1

            Components

            50T

            100T

            FL Buffer

            30ml

            60ml

            PK Solution

            0.5ml

            1ml

            WS Buffer

            5ml

            10ml

            Binding Buffer

            35ml

            70ml

            PW Buffer

            12ml

            (add 18ml ethanol before use)

            24ml

            (add 36ml ethanol before use)

            Wash Buffer

            26ml

            (add 39ml ethanol before use)

            52ml

            (add 78ml ethanol before use)

            Elution Buffer

            10ml

            20ml

            Spin Column

            50

            100

            Handbook

            1 copy

            1 copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from marine animal tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA without proteins and other contaminants. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

                The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

            Product Features:

            Storage

            • The PK solution is to be stored at 2-8℃, others at 15-25℃.
            •  All reagents, when stored properly, are stable for 18 months.

            Apparatus and Materials to Be Supplied by the User

            * Sterile 2.0ml microcentrifuge tubes                * 10µl/100µl/1000µl tips

            * Microcentrifuge capable of 14,000g                * Absolute ethanol

            Important notes

            1. Please add3.6ml absolute ethanol to PW Buffer and mix thoroughly before the first use.
            2. Please add 8.4ml absolute ethanol to Wash Binding Buffer and mix thoroughly before the first use.
            3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

            Expressions:concentration(μg/ml)=50×OD260×dilution fact

            Target:2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis 0.8~1% Agarose gel

            Example 1:Fishtail

            Example 2:Shell meat

            Manual download
          • Biospin Insect Genomic DNA Extraction Kit MORE
            Product Details:
            Kit Components

            Cat#

            BSC26S1

            BSC26M1

            Components

            50Tests

            100Tests

            FL Buffer

            30.0ml

            60.0ml

            PK solution

            1ml

            2ml

            Binding Buffer

            35.0ml

            70.0ml

            PW Buffer

            11ml(add 16.5ml ethanol before use)

            22ml(add 33ml ethanol before use)

            Wash Buffer

            25ml(add 37.5ml ethanol before use)

            50ml(add 75ml ethanol before use)

            Elution Buffer

            10.0ml

            20.0ml

            Spin Column

            50

            100

            Handbook

            1 copy

            1 copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from insect, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps. It can effectively remove all kinds of pigment, chitin, polysaccharide and other impurities. It can also extract multiple samples once.

                The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

            Product Features:

            Storage

            • The PK Solution is to be stored at 2-8℃, others at 15-25℃.
            • All reagents, when stored properly, are stable for 18 months.

            Apparatus and Materials to Be Supplied by the User

            *  Sterile 1.5/2.0ml microcentrifuge tubes          * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g               * Absolute ethanol         * Chloroform

            Important notes

            1. Please add 33ml absolute ethanol to PW Buffer and mix thoroughly before the first use.

            2. Please add 75ml absolute ethanol to wash Binding Buffer and mix thoroughly before the first use.

            3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.

             

          • Biospin Fungus Genomic DNA Extraction Kit MORE
            Product Details:

            Kit Components 

            Cat#

            BSC14S1

            BSC14M1

            Components

            50Tests

            100Tests

            LE Buffer

            20 ml

            40 ml

            DA Buffer

            7.5 ml

            15 ml

            E Binding Buffer

            14ml

            (add 28 ml absolute ethanol before use)

            28ml

            (add 56 ml absolute ethanol before use)

            G Binding Buffer

            25ml

            50ml

            Wash Buffer

            25.2ml

            (add 37.8ml absolute ethanol before use)

            50.4ml

            (add 75.6ml absolute ethanol before use)

            Elution Buffer

            10ml

            20ml

            Spin column

            50

            100

            Handbook

            1 copy

            1 copy

            Introduction

                The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from fungus, adopting Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 2.5-30 μg genomic DNA can be acquired from up to 100 mg tissue or up to 5ml yeast culture by using Biospin Fungus Genomic DNA Extraction Kit.

                The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

            Product Features:

            Storage

            • The kit should be stored at 15-25℃.
            • All reagents, when stored properly, are stable for 18 months.

            Apparatus and Materials to Be Supplied by the User

            *  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

            *  Microcentrifuge capable of 14,000g              * Absolute ethanol     * Vortex mixer

            Important notes

            1. Please add absolute ethanol to Wash Buffer and mix thoroughly before the first use.
            2. Please add absolute ethanol to E Binding Buffer and mix thoroughly before the first use.
            3. LE Buffer may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 37°C until the precipitate has fully dissolved.
            4. Prepare Sorbitol buffer: 18.217 g Sorbitol and 3.7224 g EDTA-2Na, dissolved in 100 ml of pure water.
            Experimental data:

            Analysis DNA

            • Absorbance analysis

            Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260   ,OD280 and OD320.

            Expressions:concentration(μg/ml)=50×OD260×dilution fact Target:                2.0≥OD260-320/ OD280-320≥1.7

            Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

            • Agarose Gel Analysis 0.81Agarose gel

            Example 1:

            Manual download
          • Biospin FFPE Tissue RNA Extraction Kit MORE
            Product Details:

            Kit Components 

             

            Cat#

             

             

            BSC66S1

             

             

            BSC66M1

            Components

            50Tests

            100Tests

            Deparaffinization Solution

            50ml

            100ml

            Lysis II Buffer

            10ml

            20ml

            Binding Buffer

            10ml

            20ml

            SW Buffer

            26ml

            52ml

            Wash Buffer II

            8ml

            (Add 32 ml ethanol before use)

            16ml

            (Add64 ml ethanol before use)

            RElution Buffer

            10ml

            20ml

            PK Solution

            500µl (Stored at 2-8℃)

            1ml (Stored at 2-8℃)

            Spin Column

            50

            100

            Handbook

            1 copy

            1 copy

            Cat#

            BSA35S2B (Stored at -20)

            BSA35M2B (Stored at -20)

            DNase I Buffer

            1.3 mlх2

            1.3 mlх4

            DNase I

            100µl

            200µl

            Introduction

                This kit is used to extract high- purity RNA from FFPE tissue sections, with non-toxic deparaffinization solution, high-performance Lysis  II Buffer to release RNA from FFPE  efficiently. The high efficient binding of RNA to our spin matrix while proteins and other impurities are removed by   wash buffer. Nucleic acids are easily eluted with sterile RNase free water or RElution Buffer. The purified RNA is ready for downstream applications such as Real-Time PCR,Sec

            Product Features:

            Storage and transportation

            • The kit has demonstrated stability of 12 months when the PK Solution should be stored at 2-8℃,DNase I should be stored at -20℃,others at room temperature.
            • The kit can be transported at room temperature, but PK Solution and DNase I need cold chain.

            Apparatus and materials to be prepared by the user

            *  1.5ml microcentrifuge tubes                        * 10µl/100µl/1000µl tips

            *  Centrifuge capable of ≥14,000g                 * Ethanol(≥95%)

            *  Heating block or water bath

            Important note

            1)Wash Buffer II: Add the ethanol as the volume marked on bottle label and mix well.

            2)Lysis II Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

            3)PK Solution should be stored at 2°C-8℃.

            4)DNase I should be stored at -20°C.

            5)Carry out all centrifugations at room temperature.

            Experimental data:

            Analysis RNA

            Real-Time PCR compare

            Manual download
          12
          Privacy clause

          Respect the user's privacy is a basic policy of Hangzhou Bioer user service. Hangzhou Boier will not disclosure user information to third parties or disclose their registration and preservation of non disclosure in Hangzhou Bioer platform, except in the following situations: (1)User's improper behavior caused leakage of privacy information ; (2)Caused by network, hacker attack, computer virus, government regulation, the reasons of the leakage, lost, stolen or tampered with other relevant legal requirements; (3)Law-enforcement agency asked Hangzhou Bioer to provide privacy information of the user; (4)Emergency situation when public life and property security is under threate.

          Exemption clause

          Hangzhou Bioer technology does not guarantee the accuracy, integrity and reliability of any content on this website and any content that may use these results. Hangzhou Bioer technology and related people can not guarantee that you can enter, browse and use this website at any time; Hangzhou Bioer technology on this website and its contents can not use and do not take any responsibility. The site links to other websites that Hangzhou itself is not recognized or undertake other Bioer technology content or the use of responsibility. Hangzhou Bioer technology and related people to enter, browse and use of the site, from the website to download any content or by any link to this site to other sites caused by viruses or other destructive programs on your computer system and any other software and hardware, IT system or property damage or loss does not bear any responsibility. Hangzhou Bioer technology and the relevant people do not bear any responsibility caused by the third party using illegal means to obtain the password, data and content into the site of any direct and indirect damage or loss . The final interpretation belong to Hangzhou Bioer Technology Co. Ltd.

          Hangzhou Bioer reserves the right to correct, modify and update this statement at any time.

          Copyright

          This website from Hangzhou Bioer Technology Co. Ltd. (hereinafter referred to as "Hangzhou Bioer") the creation of any content of this website (including but not limited to text, data, graphics, images, voice or video) are copyright or related rights belong to Hangzhou Bioer. Hangzhou Bioer or related rights without prior written permission, you shall not in any way without permission to copy, reconstruction, spread, publishing, reprint, repost or display the contents of this website. Any unauthorized use of this site may violate the "Copyright Law of People's Republic of China " and other relevant laws and regulations and international conventions, Hangzhou Bioer fully pursue appropriate legal responsibility right.

          Contact Us

          Address: 1192 Bin An Rd, Hi-tech (Binjiang)District,Hangzhou,310053,P. R. China

          Tel:0571-87774567

          万搏电竞max-万博电竞max-万博电竞max下载

          
          
          <address id="lxprp"></address>

          <address id="lxprp"></address>

          <form id="lxprp"></form>

                <address id="lxprp"><nobr id="lxprp"><meter id="lxprp"></meter></nobr></address>

                <em id="lxprp"><form id="lxprp"></form></em>
                  <noframes id="lxprp"><listing id="lxprp"></listing>

                  <address id="lxprp"><listing id="lxprp"><menuitem id="lxprp"></menuitem></listing></address>